The objective of this project is to determine the mechanisms that control the reinnervation of the cornea after spontaneous or induced injuries (eye surgery), as well as the role of corneal epithelial cells in this process. Using this information, we will try to implement novel therapeutic approaches that improve reinnervation and the status of the corneal epithelium after axotomy. Specifically, the efficacy of treatment with different well-known neurotrophic cytokines and with new molecules identified in this project in nerve regeneration will be tested, as well as the efficacy of treatment with these agents in maintaining a normal trophic state of the cornea.

We trust that this information can serve as the basis for designing specific treatments that preserve the state of the corneal epithelium after accidents, replacement surgery and genetic or inflammatory diseases of the peripheral nervous system.

The specific objectives are:

Objective 1. To explore the molecular and cellular mechanisms by which corneal epithelial cells contribute to axonal survival and nerve regeneration after injury.
Objective 2. To determine the efficacy of topical treatment with one or more cytokines with neurotrophic activity on the regeneration of corneal nerves after surgical corneal injury.
Objective 3. To determine the efficacy of topical treatment with one or more molecules with neurotrophic activity to maintain the trophic state of the injured cornea, as well as a normal tear rate and a normal blink reflex.
Objective 4. Obtain genetically modified mice that serve as a tool for studying the role of non-myelinating Schwann cells in the biology and pathophysiology of the cornea.